BOVINE TRANSFERRINS: SIALIC ACID AND THE COMPLEX PHENOTYPE

Involvement of sialic acid in bovine sperm-zona pellucida interaction

Role of sialic acid in bovine sperm-zona pellucida binding.

Sperm binding activity has been detected in zona pellucida (ZP) glycoproteins and it is generally accepted that this activity resides in the carbohydrate moieties. In the present study we aim to identify some of the specific carbohydrate molecules involved in the bovine sperm-ZP interaction. We performed sperm binding competition assays, in vitro fecundation (IVF) in combination with different ...

The role of sialic acid synthases in sialic acid biosynthesis

Introduction The sialic acids comprise a diverse family of nine-carbon keto-sugars, the majority of which are structural derivatives of neuraminic acid (Fig. 1). Neuraminic acid has an amino moiety at the C-5 position, and carboxylate at C-2 which confers negative charge at physiological pH. Whereas unsubstituted neuraminic acid is not known to exist in nature, over 40 substituted variations (c...

The effect of bovine viral diarrhea virus on bovine monocyte phenotype

Bovine viral diarrhea virus (BVDV) is an economically important pathogen of the livestock industry worldwide. BVDV is classified into cytopathic (cp) and noncytopathic (ncp), depending on its effects on cultured cells. BVDV is known to alter the host’s immune response. Of this, major histocompatibility complex (MHC) class II molecules play a central role in the development and function of the i...

Sialic acid and cell aggregation.

Chick embryo neural retina cells from 7-, 10and 14-day embryos and muscle cells from 9-day embryos, dissociated mechanically or with trypsin, reaggregate at the same initial rate whether treated before and during aggregation with active or inactive iV-acetyl neuraminidase. 7V-acetyl neuraminidase treatment also has no effect on the size or number of aggregates formed over 24 or 48 h of aggregat...